1,4-DITHIOTHREITOL

Dithiothreitol (DTT) is the common given name for a small-molecule redox reagent also known as Cleland's reagent with formula of C4H10O2S. An excellent reagent for maintaining SH groups in reduced state quantitatively reduces disulfides. DTT is effective in sample buffers for dropping protein disulfide bonds prior to SDS-PAGE. It is also used as a reducing or deprotecting agent for thiolated DNA and it can be used as an oxidizing agent.

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Detailed Description for 1,4-DITHIOTHREITOL

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystami​ne to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Erythritol is a  a four-carbon sugar alcohol formed from erythrose by reduction of the carbonyl group; found in algae, lichens, grasses, and certain fungi. It is it is about twice as sweet as sucrose and used as a sweetener or food additive.  But it is not absorbed by the digestive system in the body.  sugar alcohol which has been approved for use in the US as a food additive and sweetener. Erythritol (more correctly meso-erythritol; meso- means inactive) is the optically inactive isomeric tetritol among three stereoisomers. The other two stereoisomeric tetritols are D- and L-threitol. The only existing three-carbon sugar alcohol (triitol) is glycerol. The four stereoisomeric pentitols are xylitol, ribitol, and D- or L-arabitol. Mercaptan esters of threitol, low molecular weight thiols, reduces disulfide bonds quantitatively and maintains monothiols in the reduced state, which provides the applications including protein processing, induction of genes, oligonucleotide synthesis. Cleland's reagent is the commonest reagent for maintaining -SH groups in reduced state. Mercaptan esters of threitol have been studied as anti-cancer agents by acting as alkylating agents to damage the cancer cell DNA.


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Seema S

Certification Detail is necessary Posted on 8/26/2016

While purchasing only CAS No. is not sufficient, be sure about standards of certification which is necessary for safety of chemicals and person. Some of the standard certifications are ISO9001 and ISO14001.

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